14-P3N-ScG

Human Parainfluenza Virus type 3 recombinant nucleocapsid protein (hPIV3-rNP) from S. cerevisiae, in glycerol stock

Description: human Parainfluenza Virus type 3 (hPIV3) strain C-243 full-length (515 a.a.) recombinant nucleoprotein (GenBank: ABY63596.1). Expressed in S. cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.

Quality: assembled in nucleocapsid-like particles (NLPs), which are morphologically similar to native viral nucleocapsids.

Current Lot EM picture: hPIV3-rNP preparation visualized by negative staining with uranyl acetate, magnification 180.000x (maximal)

Form: formulated in glycerol stock solution (50%) in phosphate buffer.

Storage: Shipped on dry ice (shipment costs to Europe: 160 €). Store at -20ºC in liquid form (solution does not freeze at this temperature).

Concentration: 1.4 mg/ml.

Purity: >93% as determined by SDS-PAGE.

SDS-PAGE showing hPIV3-rNP product 14-P3N-ScG at approximately 65 kDa (2.8 μg/lane).

Applications (tested): ELISA, Electron microscopy, Western blot, SDS-PAGE.

Unit/Price:

Reference:

1. Juozapaitis M, Zvirbliene A, Kucinskaite I, Sezaite I, Slibinskas R, Coiras M, de Ory Manchon F, López-Huertas MR, Pérez-Breña P, Staniulis J, Narkeviciute I, Sasnauskas K: Synthesis of recombinant human parainfluenza virus 1 and 3 nucleocapsid proteins in yeast Saccharomyces cerevisiae. Virus Res. 2008 May;133(2):178-86.
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