Mouse monoclonal antibody (cl. 11H7) to Human Parainfluenza Virus type 3 nucleocapsid protein
Description: Mouse monoclonal antibody (clone 11H7) to Human Parainfluenza Virus type 1 nucleocapsid protein. Primary antibody, unconjugated.
Immunogen: Human Parainfluenza Virus type 3 recombinant nucleocapsid protein expressed in S. cerevisiae, hPIV3-rNP (product code 14-P3N-Sc).
Tested applications: ELISA, Western blot (WB), immunofluorescence (IF)
Western blot using monoclonal antibody 11H7 to Human Parainfluenza Virus type 3 nucleocapsid protein (Cat. code 18MAk-hPIV3-11H7), 5 µg/ml, followed by secondary anti-mouse antibody conjugated to HRP.
M – PageRuler™ Prestained Protein Ladder, 10 to 180 kDa, Thermo Fisher Scientific,
1 – hPIV3-rNP, Human Parainfluenza Virus type 3 nucleocapsid protein expressed in S. cerevisiae (14-P3N-ScG), 1 µg,
2 – hPIV1-rNP, Human Parainfluenza Virus type 1 nucleocapsid protein expressed in S. cerevisiae (14-P1N-ScG), 1 µg,
3 – S. cerevisiae lysate, 10 µg,
4 – P. pastoris lysate, 10 µg.
Proteins were separated in 12% SDS-PAGE.
Chromogenic detection was performed by 4-chloro-1-naphthol.
|Immunofluorescence staining of hPIV-3 infected vero cells
(Mosaic Parainfluenza virus types 1 – 4, Euroimmun)
with monoclonal antibody 11H7 (Cat. code 18MAk-hPIV3-11H7).
Recommended starting dilutions: WB 1/200-1/1000; IF 1/20-1/200; ELISA 1/1000-1/10000. Optimal concentrations should be determined by the end user.
Purity: Protein A purified
Form: PBS with 0.1% sodium azide
Concentration: 1 mg/ml
Storage conditions: This product is stable at +2 – +8°C for 2-3 weeks. For a long-term storage the antibody can be stored at -20°C or -80°C (avoid freeze / thaw cycle). Alternatively, in 50% glycerol at -20°C.
Conditions of shipping: Blue ice (0-8°C). Shipment cost: 70 €.
Additional info: The epitope for Human Parainfluenza Virus type 3 nucleocapsid protein recognised by antibody 11H7 is located between 437 and 446 aa (protein Accession No. in GenBank: ABY63596.1). The antibody 11H7 reacts with biotinylated synthetic peptide GEPQSSIIQY corresponding to Human Parainfluenza Virus type 3 nucleocapsid protein sequence (protein Accession No. in GenBank: ABY63596.1). It does not cross-react with Human Parainfluenza Virus type 1 nucleocapsid protein.
Availability: can be prepared for shipment in 2 weeks.
|100 μg (100 μl)||18MAk-hPIV3-11H7-C||300 €||Inquiry / Order
|500 μg||18MAk-hPIV3-11H7-D||1250 €|
|1 mg||18MAk-hPIV3-11H7-M||2000 €|
+Shipment cost: 70 €.
1. Zvirbliene A, Sezaite I, Pleckaityte M, Kucinskaite-Kodze I, Juozapaitis M, Sasnauskas K: Mapping of an Antigenic Site on the Nucleocapsid Protein of Human Parainfluenza Virus Type 3. Viral Immunology, 22(3) (2009) 181-188. doi: 10.1089/vim.2008.0106.