Human Parainfluenza Virus type 1 recombinant nucleocapsid protein (hPIV1-rNP) from S. cerevisiae, lyophilized

Description: human Parainfluenza Virus type 1 (hPIV1) strain C-35 full-length (524 a.a.) recombinant nucleoprotein (GenBank: ABY63595.1). Expressed in S. cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.

Form: Lyophilized in PBS.

Storage: Shipped at ambient temperature (shipment costs: 65 € to Europe and 100 € to US). Lyophilized products may be stored at 2-8°C or at -20°C for longer periods.

Reconstitution: add 100 μl of deionized water for reconstitution of 100 µg of hPIV1-rNP in each separate vial. Check lyophilized material to be completely dissolved before use.

Concentration of reconstituted product: 1.0 mg/ml in PBS.

Purity: >90% as determined by SDS-PAGE.

14-P1N-ScL1-LotPI101-1412

SDS-PAGE analysis showing hPIV1-rNP product 14-P1N-ScL at approximately 58 kDa (3 μg/lane).

Applications (tested): ELISA, other immunoassays, Western blot, SDS-PAGE.

Unit/Price:

Size Catalogue No. Price
100 μg 14-P1N-ScL-C 175 € Inquiry /  Order
product
500 μg (5×100 μg) 14-P1N-ScL-D 650 €
1 mg (10×100 μg) 14-P1N-ScL-M 1000 €

 

Reference:

  1. Juozapaitis M, Zvirbliene A, Kucinskaite I, Sezaite I, Slibinskas R, Coiras M, de Ory Manchon F, López-Huertas MR, Pérez-Breña P, Staniulis J, Narkeviciute I, Sasnauskas K: Synthesis of recombinant human parainfluenza virus 1 and 3 nucleocapsid proteins in yeast Saccharomyces cerevisiae. Virus Res. 2008 May;133(2):178-86.
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