Measles virus recombinant nucleoprotein (Me-rN) from S. cerevisiae, lyophilized

Description: Measles virus strain Edmonston (Schwarz vaccine) full-length (525 a.a.) recombinant nucleoprotein (GenBank: AAF85699.1). Expressed in Saccharomyces cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.

Form: Lyophilized in PBS.

Storage: Shipped at ambient temperature (shipment costs to Europe: 70 €). Lyophilized products may be stored at 2-8°C or at -20°C for longer periods.

Reconstitution: add 133 μl of deionized water for reconstitution of 200 µg of Me-rN protein in each separate vial. Check lyophilized material to be completely dissolved before use.

Concentration of reconstituted product: 1.5 mg/ml in PBS.

Purity: >90% as determined by SDS-PAGE.


SDS-PAGE showing reconstituted Me-rN product 12MeN-BSc-L at approximately 58 kDa (3 μg/lane).

Applications (tested): Western blot, SDS-PAGE.

Note: has not been tested yet for other applications.


Size Catalogue No. Price
200 μg 12MeN-BSc-L-CC 375 € Inquiry / Order
600 μg (3×200 μg) 12MeN-BSc-L-DC 950 €
1 mg (5×200 μg) 12MeN-BSc-L-M 1250 €



  1. Samuel D, Sasnauskas K, Jin L, Gedvilaite A, Slibinskas R, Beard S, Zvirbliene A, Oliveira SA, Staniulis J, Cohen B, Brown D: Development of a measles specific IgM ELISA for use with serum and oral fluid samples using recombinant measles nucleoprotein produced in Saccharomyces cerevisiae. J Clin Virol. 2003 Oct; 28(2):121-9.
    Full text PDF.
  2. Slibinskas R, Samuel D, Gedvilaite A, Staniulis J, Sasnauskas K: Synthesis of the measles virus nucleoprotein in yeast Pichia pastoris and Saccharomyces cerevisiae. J Biotechnol. 2004; 107(2):115-124.
    Full text PDF.