20-S2RBD-RCg-F

SARS-CoV-2 Spike glycoprotein Receptor Binding Domain from mammalian CHO cells, frozen

Description: SARS-CoV-2 Spike glycoprotein Receptor Binding Domain (SCoV2-RBD) expressed as secreted protein in mammalian (hamster) CHO cells. The construct contains amino acids 319-529 of the Spike glycoprotein provided in UniProtKB sequence accession number: P0DTC2 (SPIKE_SARS2). Whole expression construct includes SCoV2-RBD (aa 319 – 529 of Spike glycoprotein) followed by introduced protease cleavage site and C-terminal His6-tag. SCoV2-RBD protein was purified from culture medium under native (non-denaturing) conditions using convenient chromatographic techniques. Then it was dialyzed against PBS, adjusted to 1.0 mg/ml concentration, filter-sterilized, aliquoted and frozen for storage.

Form: Frozen liquid in PBS (pH 7.4).

Storage: The product is shipped with dry ice (shipment cost to Europe: 160 €). Store at -80°C for the long term. After thawing may be stored for short-term at 4°C (stable at least 2 weeks at this temperature). Avoid repeated freeze/thaw cycles.

Concentration: 1.0 mg/ml.

Purity: >95 % as determined by SDS-PAGE.

Image: SDS-PAGE showing SCoV2-RBD product 20-S2RBD-RCg-F at approximately 30-33 kDa (1, 3 and 5 μg/lane).

Applications (tested): ELISA, other immunoassays, Western blot, SDS-PAGE.

Reactivity of SCoV2-RBD protein (current batch of product 20-S2RBD-RCg-F) with specific IgG in ELISA using pools of Covid-19 positive and negative sera:

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