SARS-CoV-2 recombinant Spike (SCoV2-rS) glycoprotein full-length trimeric ectodomain and mutant variants of SCoV2-S

SARS-CoV-2 recombinant Spike (SCoV2-rS) glycoprotein full-length ectodomain expressed as secreted trimeric protein in mammalian cells. It was purified from culture medium under native (non-denaturing) conditions using convenient chromatographic techniques. Six different products of initial sequence (Wuhan) SCoV2-rS protein are available:

Note: The same protein from different cell lines is not identical in respect of post-translational modifications including glycosylation. Protein from CHO cells is more uniformly glycosylated and to a lesser extent than analogous product from HEK293 cells. Nevertheless, Spike glycoproteins secreted from either CHO or HEK cells show similar results in ELISA with COVID-19 positive and negative human sera.

Also, main Spike protein mutants (alpha, beta, delta, omicron, etc. variants) were synthesized in a form of the full-length trimeric proteins in CHO cells and purified in similar way as the initial SCoV2-rS protein sequence variant. Formulations of five mutants in either PBS (frozen) or glycerol stocks are available:

SARS-CoV-2 Spike glycoprotein Receptor Binding Domain (SCoV2-RBD)

SARS-CoV-2 recombinant Spike glycoprotein Receptor Binding Domain (SCoV2-RBD) expressed as secreted protein in mammalian cells. It was purified from culture medium under native (non-denaturing) conditions using convenient chromatographic techniques. Five different products are available:

 

Note: The same protein from different cell lines is not identical in respect of glycosylation. Protein from CHO cells is glycosylated at lesser extent than analogous product from HEK293 cells. SCoV2-RBD secreted from either CHO or HEK cells showed similar results in ELISA with COVID-19 positive and negative human sera.