14-IAH-H2L

Highly reactive his-tagged Influenza A Virus recombinant nucleoprotein (IAV-His8-rN) from S. cerevisiae, lyophilized

Description: Influenza A virus (IAV) strain A/New York/384/2005(H3N2) full-length (498 a.a.) recombinant Nucleoprotein (UniProtKB/Swiss-Prot sequence accession number: Q3YQ35) with His8-tag on the N-terminus. Expressed in S. cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions. Reactivity of this protein product in immunoassays was improved.

Form: Lyophilized in PBS with 3 mg/ml sucrose.

Storage: Shipped at ambient temperature (shipment costs: 65 € to Europe and 100 € to US). Lyophilized products may be stored at 2-8°C or at -20°C for longer periods.

Reconstitution: add 100 μl of deionized water for reconstitution of 100 µg of IAV-his8-rN protein in each separate vial. Check lyophilized material to be completely dissolved before use.

Concentration of reconstituted product: 1.0 mg/ml in PBS.

Purity: ~85% as determined by SDS-PAGE.

SDS-PAGE analysis showing IAV-rN product 14-IAH-H2L at approximately 57 kDa (3 μg/lane).

Applications (tested): ELISA and other immunoassays (improved reactivity), Western blot, SDS-PAGE.

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