18MAk-hPIV3-2H1

Mouse monoclonal antibody (cl. 2H1) to Human Parainfluenza Virus type 3 nucleocapsid protein

Description: Mouse monoclonal antibody (clone 2H1) to Human Parainfluenza Virus type 1 nucleocapsid protein. Primary antibody, unconjugated.

Clone: 2H1

Host: mouse

Subclass: IgG1

Immunogen: Human Parainfluenza Virus type 3 recombinant nucleocapsid protein expressed in S. cerevisiae, hPIV3-rNP (product code 14-P3N-Sc).

Tested applications: ELISA, Western blot (WB), immunofluorescence (IF)

Western blot using monoclonal antibody 2H1 to Human Parainfluenza Virus type 3 nucleocapsid protein (Cat. code 18MAk-hPIV3-2H1), 5 µg/ml, followed by secondary anti-mouse antibody conjugated to HRP.

M – PageRuler™ Prestained Protein Ladder, 10 to 180 kDa, Thermo Fisher Scientific,
1 – hPIV3-rNP, Human Parainfluenza Virus type 3 nucleocapsid protein expressed in S. cerevisiae (14-P3N-ScG), 1 µg,
2 – hPIV1-rNP, Human Parainfluenza Virus type 1 nucleocapsid protein expressed in S. cerevisiae (14-P1N-ScG), 1 µg,
3 – S. cerevisiae lysate, 10 µg,
4 – P. pastoris lysate, 10 µg.

Proteins were separated in 12% SDS-PAGE.
Chromogenic detection was performed by 4-chloro-1-naphthol.

Immunofluorescence staining of hPIV-3 infected vero cells (Mosaic Parainfluenza virus types 1 – 4, Euroimmun) with monoclonal antibody 2H1 (Cat. code 18MAk-hPIV3-2H1).

Recommended starting dilutions: WB 1/200-1/1000; IF 1/20-1/200; ELISA 1/1000-1/10000. Optimal concentrations should be determined by the end user.

Purity: Protein A purified

Form: PBS with 0.1% sodium azide

Concentration: 1 mg/ml

Storage conditions: This product is stable at +2 – +8°C for 2-3 weeks. For a long-term storage the antibody can be stored at -20°C or -80°C (avoid freeze / thaw cycle). Alternatively, in 50% glycerol at -20°C.

Conditions of shipping: Blue ice (0-8°C). Shipment cost: 70 €.

Additional info: The epitope for Human Parainfluenza Virus type 3 nucleocapsid protein recognised by monoclonal antibody 2H1 is located between 417 and 456 aa (Protein Accession No. in GenBank: ABY63596.1). It does not cross-react with Human Parainfluenza Virus type 1 nucleocapsid protein.

Availability: can be prepared for shipment in 2 weeks.

Unit/Price:

+Shipment cost: 70 €.

Reference:

1. Zvirbliene A, Sezaite I, Pleckaityte M, Kucinskaite-Kodze I, Juozapaitis M, Sasnauskas K: Mapping of an Antigenic Site on the Nucleocapsid Protein of Human Parainfluenza Virus Type 3. Viral Immunology, 22(3) (2009) 181-188. doi: 10.1089/vim.2008.0106.