18MAk-HEV-5F3

Mouse monoclonal antibody (cl. 5F3) to Hepatitis E Virus genotype 3 capsid protein

Description: Mouse monoclonal antibody (clone 5F3) to hepatitis E virus genotype 3 capsid protein. Primary antibody, unconjugated.

Clone: 5F3

Host: mouse

Subclass: IgG2b

Immunogen: S.cerevisae – expressed hepatitis E virus genotype 3 (isolate wbGER27, GenBank FJ705359) full-length capsid protein, HEV3-rCP.

Tested applications: ELISA, Western blot (WB), immunofluorescence (IF), hepatitis E virus (genotype 3) neutralisation test.

Western blot using monoclonal antibody 5F3 to hepatitis E virus capsid protein (Cat. code 18MAk-HEV-5F3), 0.5 µg/ml, followed by secondary anti-mouse antibody conjugated to HRP.

M – PageRuler™ Prestained Protein Ladder, 10 to 180 kDa, Thermo Fisher Scientific,
1 – purified rat hepatitis E virus (genotype C1) capsid protein produced in S. cerevisiae, 0.5 µg,
2 – HEV3-rCP, purified HEV (genotype 3) capsid protein produced in S. cerevisiae, 0.5 µg,
3 – S. cerevisiae lysate, 10 µg.

Proteins were separated in 12% SDS-PAGE.
Chromogenic detection was performed by 4-chloro-1-naphthol.

Immunofluorescence staining of hepatitis E virus genotype 3 (strain 47832c) infected A549 cells with monoclonal antibody 5F3 (Cat. code 18MAk-HEV-5F3).

Recommended starting dilutions: ELISA – 1:1000 to 1:10000, WB – 1:500 to 1:2000, IF – 1:20 to 1:50. Optimal concentrations should be determined by the end user.

Purity: Protein A purified

Form: PBS with 0.1% sodium azide

Concentration: 1 mg/ml

Storage conditions: This product is stable at +2 – +8°C for 2-3 weeks. For a long-term storage the antibody can be stored at -20°C or -80°C (avoid freeze / thaw cycle). Alternatively, in 50% glycerol at -20°C.

Conditions of shipping: Blue ice (0-8°C). Shipment cost: 70 €.

Additional info: –

Availability: can be prepared for shipment in 2 weeks.

Unit/Price:

+Shipment cost: 70 €.

Reference:

1. Simanavicius M, Tamosiunas PL, Petraityte-Burneikiene R, Johne R, Ulrich RG, Zvirbliene A, Kucinskaite-Kodze I: Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles. Appl Microbiol Biotechnol. 2018 Jan;102(1):185-198. doi: 10.1007/s00253-017-8622-9.