12MeN-BPp-Gly

Measles virus recombinant nucleoprotein (Me-rN) from P. pastoris, in glycerol stock

Description: Measles virus strain Edmonston (Schwarz vaccine) full-length (525 a.a.) recombinant nucleoprotein (GenBank: AAF85699.1). Expressed in Pichia pastoris and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.

Quality: assembled in nucleocapsid-like particles (NLPs), which are morphologically similar to native viral nucleocapsids.

Current Lot EM picture: Me-rN preparation visualized by negative staining with uranyl acetate, magnification 89.000x

Form: formulated in glycerol stock solution (50%) in phosphate buffer.

Storage: Shipped on dry ice (shipment costs: 130 € to Europe and 150 € to US). Store at -20ºC in liquid form (solution does not freeze at this temperature).

Concentration: 1.5 mg/ml.

Purity: >90% as determined by SDS-PAGE.

SDS-PAGE showing Me-rN product 12MeN-BPp-Gly at approximately 58 kDa (3 μg/lane).

Applications (tested): ELISA, other immunoassays, Electron microscopy, Western blot, SDS-PAGE.

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References:
1. Slibinskas R, Samuel D, Gedvilaite A, Staniulis J, Sasnauskas K: Synthesis of the measles virus nucleoprotein in yeast Pichia pastoris and Saccharomyces cerevisiae. J Biotechnol. 2004 Jan 22;107(2):115-24.
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2. Warrener L, Slibinskas R, Chua KB, Nigatu W, Brown KE, Sasnauskas K, Samuel D, Brown D: A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid. Bull World Health Organ. 2011 Sep 1;89(9):675-82.

3. Shonhai A, Warrener L, Mangwanya D, Slibinskas R, Brown K, Brown D, Featherstone D, Samuel D: Investigation of a measles outbreak in Zimbabwe, 2010: potential of a point of care test to replace laboratory confirmation of suspected cases. Epidemiol Infect. 2015 Dec;143(16):3442-50.