13ERp57-EPp-F

Native recombinant human ERp57 from P. pastoris, frozen

Description: Human ERp57 (PDIA3) full length recombinant mature protein (481 a.a.; 25-505 a.a.) (UniProtKB/Swiss-Prot sequence accession number: P30101). Expressed in P. pastoris as a full length precursor (1-505 a.a.), which is processed in to the mature form (signal peptide (1-24 a.a.) is cleaved after translocation in to the ER) and secreted in to the yeast growth medium. Recombinant ERp57 is purified from the yeast growth medium using convenient chromatographic techniques.

Quality: Corresponds to native human ERp57 protein in so far as possible. Does not contain any additional amino acids or posttranslational modifications. Exhibits thiol-dependent reductase activity which catalyzes the reduction of insulin disulfides by dithiothreitol.

Form: Stored in -70˚C in 20 mM TRIS, pH 8.0, 100 mM NaCl.

Storage: Shipped on dry ice (shipment costs: 130 € to Europe and 150 € to US). Protein is stable for 1-2 weeks at 2-8°C. For longer periods should be stored at -70°C.

Concentration: 2.3 mg/ml.

Purity: >90% as determined by SDS-PAGE.

SDS-PAGE analysis showing human ERp57 product 13ERp57-EPp-F at approximately 55 kDa (5 μg/lane).

Applications (tested): Functional assays, western blot and SDS-PAGE.

Unit/Price:

References:

Čiplys E, Žitkus E, Slibinskas R: Native signal peptide of human ERp57 disulfide isomerase mediates secretion of active native recombinant ERp57 protein in yeast Saccharomyces cerevisiae. Protein Expr Purif. 2013 Jun;89(2):131-5.
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