14-IAUlt-U1L

Ultrapure Influenza A Virus recombinant nucleoprotein (IAV-rN) from S. cerevisiae, lyophilized

Description: Influenza A virus (IAV) strain A/New York/384/2005(H3N2) full-length (498 a.a.) recombinant Nucleoprotein (UniProtKB/Swiss-Prot sequence accession number: Q3YQ35). Expressed in S. cerevisiae and purified from yeast lysates by combination of chromatographic techniques and ultracentrifugation in CsCl gradients under native (non-denaturing) conditions to ~95% purity.

Form: Lyophilized in PBS.

Storage: Shipped at ambient temperature (shipment costs: 65 € to Europe and 100 € to US). Lyophilized products may be stored at 2-8°C or at -20°C for longer periods.

Reconstitution: add 100 μl of deionized water for reconstitution of 100 µg of IAV-rN protein in each separate vial. Check lyophilized material to be completely dissolved before use.

Concentration of reconstituted product: 1.0 mg/ml in PBS.

Purity: highly pure (~95%) as determined by SDS-PAGE.

SDS-PAGE analysis showing IAV-rN product 14-IAUlt-U1L at approximately 56 kDa (3 μg/lane).

Note on processing of IAV-rN in yeast: According to ESI-MS (electrospray ionization mass spectrometry) analysis, yeast-expressed IAV-rN is N-terminally acetylated 2-498 aa protein, which is also found in native virus (see Hutchinson et al, PLoS Pathog. 2012;8(11):e1002993, Table 3).

Applications (tested): ELISA and other immunoassays (moderate reactivity), ESI-MS, Western blot, SDS-PAGE.

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