Mumps virus recombinant nucleoprotein (Mu-rNP) from S. cerevisiae, in glycerol stock
Description: Mumps virus wild-type strain Glouc1/UK96 full-length (549 a.a.) recombinant nucleoprotein (GenBank: AAG37826.1). Expressed in Saccharomyces cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.
Form: formulated in glycerol stock solution (50%) in phosphate buffer.
Storage: Shipped on dry ice (shipment costs: 130 € to Europe and 150 € to US). Store at -20ºC in liquid form (solution does not freeze at this temperature).
Concentration: 1.0-1.2 mg/ml (lot-specific).
Purity: ~90% as determined by SDS-PAGE.
SDS-PAGE showing Mu-rNP product 12MuNP-ASc-Gly at approximately 66 kDa (1.2 μg/lane).
Applications (tested): ELISA, other immunoassays, Western blot, SDS-PAGE.
Comments: According to the buoyant density in CsCl gradient (~1.29 g/ml) this protein product is present in nucleocapsid-like particles (NLPs). For more information on the S. cerevisiae-expressed mumps nucleoprotein properties see the paper of Samuel et al., 2002 (J Med Virol. 2002, 66(1):123-130).
- Samuel D, Sasnauskas K, Jin L, Beard S, Zvirbliene A, Gedvilaite A, Cohen B: High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. J Med Virol. 2002; 66(1):123-130.
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