Product Description

Mouse monoclonal antibody (cl. 7B10) to mumps virus nucleocapsid protein

Description: Mouse monoclonal antibody (clone 7B10) to mumps virus nucleoprotein. Primary antibody, unconjugated.

Clone: 7B10

Host: mouse

Subclass: IgG2a

Immunogen: mumps virus recombinant nucleocapsid protein protein expressed in S. cerevisiae, Mu-rNP (product code 12MuNP-ASc).

Tested applications: ELISA, Western blot (WB).

Western blot using monoclonal antibody 7B10 to mumps virus nucleocapsid protein (Cat. code 18MAk-Mu-7B10), 5 µg/ml, followed by secondary anti-mouse antibody conjugated to HRP.

M – PageRuler™ Prestained Protein Ladder, 10 to 180 kDa, Thermo Fisher Scientific,
1 – Mu-rNP, mumps virus recombinant nucleocapsid protein expressed in S. cerevisiae (12MuNP-ASc-Gly), 1 µg,
2 – Mu-rNP, mumps virus recombinant nucleocapsid protein expressed in P. pastoris (12MuNP-APp-Gly), 1 µg,
3 – Me-rN, measles virus recombinant nucleoprotein expressed in S. cerevisiae (12MeN-BSc-Gly), 1 µg,
4 – Me-rN, measles virus recombinant nucleoprotein expressed in P. pastoris (12MeN-BPp-Gly), 1 µg,
5 – S. cerevisiae lysate, 10 µg,
6 – P. pastoris lysate, 10 µg.

 

Proteins were separated in 12% SDS-PAGE.
Chromogenic detection was performed by 4-chloro-1-naphthol.

Recommended starting dilutions: WB 1/200-1/1000; ELISA 1/1000-1/10000. Optimal concentrations should be determined by the end user.

Purity: Protein A purified

Form: PBS with 0.1% sodium azide

Concentration: 1 mg/ml

Storage conditions: This product is stable at +2 – +8°C for 2-3 weeks. For a long-term storage the antibody can be stored at -20°C or -80°C (avoid freeze / thaw cycle). Alternatively, in 50% glycerol at -20°C.

Conditions of shipping: Blue ice (0-8°C). Shipment costs: 70 €.

Additional info:

Availability: can be prepared for shipment in 2 weeks.

Product inquiry if unable to pay with a credit card

Unit/Price:

Size Catalogue No. Price
100 μg (100 μl) 18MAk-Mu-7B10-C 300 € Inquiry / Order
product
500 μg 18MAk-Mu-7B10-D 1250 €
1 mg 18MAk-Mu-7B10-M 2000 €

+Shipment cost: 70 €.

 

Reference:
1. Samuel D, Sasnauskas K, Jin L, Beard S, Zvirbliene A, Gedvilaite A, Cohen B: High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. J Med Virol. 2002; 66(1):123-130.

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