Measles virus recombinant nucleoprotein (Me-rN) from S. cerevisiae, lyophilized
Description: Measles virus strain Edmonston (Schwarz vaccine) full-length (525 a.a.) recombinant nucleoprotein (GenBank: AAF85699.1). Expressed in Saccharomyces cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.
Form: Lyophilized in PBS.
Storage: Shipped at ambient temperature (shipment costs: 65 € to Europe and 100 € to US). Lyophilized products may be stored at 2-8°C or at -20°C for longer periods.
Reconstitution: add 133 μl of deionized water for reconstitution of 200 µg of Me-rN protein in each separate vial. Check lyophilized material to be completely dissolved before use.
Concentration of reconstituted product: 1.5 mg/ml in PBS.
Purity: >90% as determined by SDS-PAGE.
SDS-PAGE showing reconstituted Me-rN product 12MeN-BSc-L at approximately 58 kDa (3 μg/lane).
Applications (tested): Western blot, SDS-PAGE.
Note: has not been tested yet for other applications.
- Samuel D, Sasnauskas K, Jin L, Gedvilaite A, Slibinskas R, Beard S, Zvirbliene A, Oliveira SA, Staniulis J, Cohen B, Brown D: Development of a measles specific IgM ELISA for use with serum and oral fluid samples using recombinant measles nucleoprotein produced in Saccharomyces cerevisiae. J Clin Virol. 2003 Oct; 28(2):121-9.
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- Slibinskas R, Samuel D, Gedvilaite A, Staniulis J, Sasnauskas K: Synthesis of the measles virus nucleoprotein in yeast Pichia pastoris and Saccharomyces cerevisiae. J Biotechnol. 2004; 107(2):115-124.
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