Product Description

Measles virus recombinant nucleoprotein (Me-rN) from S. cerevisiae, in glycerol stock

Description: Measles virus strain Edmonston (Schwarz vaccine) full-length (525 a.a.) recombinant nucleoprotein (GenBank: AAF85699.1). Expressed in Saccharomyces cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.

Quality: assembled in nucleocapsid-like particles (NLPs), which are morphologically similar to native viral nucleocapsids.

Current Lot EM picture: Me-rN preparation visualized by negative staining with uranyl acetate, magnification 71.000x

Form: formulated in glycerol stock solution (50%) in phosphate buffer.

Storage: Shipped on dry ice (shipment costs: 130 € to Europe and 150 € to US). Store at -20ºC in liquid form (solution does not freeze at this temperature).

Concentration: 1.7 mg/ml

Purity: >90 % as determined by SDS-PAGE.

 

12MeN-BSc-Gly-LotB04

SDS-PAGE showing Me-rN product 12MeN-BSc-Gly at approximately 58 kDa (3.4 μg/lane).

Applications (tested): ELISA, other immunoassays, Electron microscopy, Western blot, SDS-PAGE.

 

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References:

  1. Samuel D, Sasnauskas K, Jin L, Gedvilaite A, Slibinskas R, Beard S, Zvirbliene A, Oliveira SA, Staniulis J, Cohen B, Brown D: Development of a measles specific IgM ELISA for use with serum and oral fluid samples using recombinant measles nucleoprotein produced in Saccharomyces cerevisiae. J Clin Virol. 2003 Oct; 28(2):121-9.
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  2. Slibinskas R, Samuel D, Gedvilaite A, Staniulis J, Sasnauskas K: Synthesis of the measles virus nucleoprotein in yeast Pichia pastoris and Saccharomyces cerevisiae. J Biotechnol. 2004; 107(2):115-124.
    Full text PDF.