Measles virus recombinant nucleoprotein (Me-rN) from S. cerevisiae, in glycerol stock
Description: Measles virus strain Edmonston (Schwarz vaccine) full-length (525 a.a.) recombinant nucleoprotein (GenBank: AAF85699.1). Expressed in Saccharomyces cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.
Quality: assembled in nucleocapsid-like particles (NLPs), which are morphologically similar to native viral nucleocapsids.
Current Lot EM picture: Me-rN preparation visualized by negative staining with uranyl acetate, magnification 71.000x
Form: formulated in glycerol stock solution (50%) in phosphate buffer.
Storage: Shipped on dry ice (shipment costs: 130 € to Europe and 150 € to US). Store at -20ºC in liquid form (solution does not freeze at this temperature).
Concentration: 1.7 mg/ml
Purity: >90 % as determined by SDS-PAGE.
SDS-PAGE showing Me-rN product 12MeN-BSc-Gly at approximately 58 kDa (3.4 μg/lane).
Applications (tested): ELISA, other immunoassays, Electron microscopy, Western blot, SDS-PAGE.
- Samuel D, Sasnauskas K, Jin L, Gedvilaite A, Slibinskas R, Beard S, Zvirbliene A, Oliveira SA, Staniulis J, Cohen B, Brown D: Development of a measles specific IgM ELISA for use with serum and oral fluid samples using recombinant measles nucleoprotein produced in Saccharomyces cerevisiae. J Clin Virol. 2003 Oct; 28(2):121-9.
Full text PDF.
- Slibinskas R, Samuel D, Gedvilaite A, Staniulis J, Sasnauskas K: Synthesis of the measles virus nucleoprotein in yeast Pichia pastoris and Saccharomyces cerevisiae. J Biotechnol. 2004; 107(2):115-124.
Full text PDF.