Human Respiratory Syncytial Virus recombinant nucleoprotein (hRSV-rN) from S. cerevisiae, lyophilized
Description: human Respiratory Syncytial Virus (hRSV) A2 strain (the major antigenic subgroup A) full-length (391 a.a.) recombinant nucleoprotein (GenBank: AAB59852.1). Expressed in S. cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.
Form: Lyophilized in PBS.
Storage: Shipped at ambient temperature (shipment costs: 65 € to Europe and 100 € to US). Lyophilized products may be stored at 2-8°C or at -20°C for longer periods.
Reconstitution: add 100 μl of deionized water for reconstitution of 100 μg of hRSV-rN protein in each separate vial. Check lyophilized material to be completely dissolved before use.
Concentration of reconstituted product: 1.0 mg/ml in PBS.
Purity: ~90% as determined by SDS-PAGE.
SDS-PAGE analysis showing hRSV-rN product 14RSV-RSc-L at approximately 40 kDa (4 μg/lane).
Applications (tested): ELISA, other immunoassays, Western blot, SDS-PAGE.