Product Description

Human Parainfluenza Virus type 3 recombinant nucleocapsid protein (hPIV3-rNP) from S. cerevisiae, lyophilized

Description: human Parainfluenza Virus type 3 (hPIV3) strain C-243 full-length (515 a.a.) recombinant nucleoprotein (GenBank: ABY63596.1). Expressed in S. cerevisiae and purified from yeast lysates by ultracentrifugation in CsCl gradients under native (non-denaturing) conditions.

Form: Lyophilized in PBS.

Storage: Shipped at ambient temperature (shipment costs: 65 € to Europe and 100 € to US). Lyophilized products may be stored at 2-8°C or at -20°C for longer periods.

Reconstitution: add 100 μl of deionized water for reconstitution of 100 µg of hPIV3-rNP in each separate vial. Check lyophilized material to be completely dissolved before use.

Concentration of reconstituted product: 1.0 mg/ml in PBS.

Purity: >93% as determined by SDS-PAGE.

14P3N-ScLiof-3mkg

SDS-PAGE analysis showing hPIV3-rNP product 14-P3N-ScL at approximately 65 kDa (3 μg/lane).

Applications (tested): ELISA, other immunoassays, Western blot, SDS-PAGE.

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Unit/Price:

Size Catalogue No. Price
100 μg 14-P3N-ScL-C 175 €  Inquiry / Order
product
500 μg 14-P3N-ScL-D 650 €
1 mg 14-P3N-ScL-M 1000 €


Reference:

  1. Juozapaitis M, Zvirbliene A, Kucinskaite I, Sezaite I, Slibinskas R, Coiras M, de Ory Manchon F, López-Huertas MR, Pérez-Breña P, Staniulis J, Narkeviciute I, Sasnauskas K: Synthesis of recombinant human parainfluenza virus 1 and 3 nucleocapsid proteins in yeast Saccharomyces cerevisiae. Virus Res. 2008 May;133(2):178-86.
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